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  • 小鼠抗ANAPC11單克隆抗體

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    • 品牌 : 通蔚生物
    • 目錄號(hào) : TW6227
    • 應(yīng)用 : 僅限于科研使用
    • 保存條件 : 低溫保存
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中文名稱(chēng):小鼠抗ANAPC11單克隆抗體

英文名稱(chēng): Anti-ANAPC11 mouse monoclonal antibody

別       名: APC11; Apc11p; HSPC214

相關(guān)類(lèi)別: 一抗

儲(chǔ)       存: 冷凍(-20℃) 避光

宿       主: Mouse

抗       原: ANAPC11

反應(yīng)種屬: Human

標(biāo)  記  物: Unconjugate

克隆類(lèi)型: mouse monoclonal



技術(shù)規(guī)格

Background:

Comprising more than ten subunits, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to APC and activates APC’s cyclin-ubiquitination activity. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC; thus preventing APC activation. APC11 is a RING-H2 finger protein that allows for the synthesis of multiubiquitin chains in the presence of Ubiquitin carrier protein 4 (Ubc4) and ubiquitin conjugating enzyme (E2). In addition, a heterodimeric complex of either Ubc4 or UbcH10 with APC11 and APC2 catalyzes the ubiquitination of human securin and cyclin B1.

Applications:

WB, IHC, IF

Name of antibody:

ANAPC11

Immunogen:

Fusion protein of human ANAPC11

Full name:

anaphase promoting complex subunit 11 (ANAPC11), transcript variant 1

Synonyms:

APC11; Apc11p; HSPC214

SwissProt:

Q9NYG5

IHC positive control:

carcinoma of human lung tissue and human pancreas tissue; human kidney tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

10 kDa

WB Positive control:

COS7 cell lysate

WB Recommended dilution:

200-1000

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